Bio Enzyme Lab

Enzyme Lab Experiments puzzle How can we demonst estimate how enzymes name? What happens if we transfer the environment of an enzyme? Materials Glucose discharge StripsTest TubesPipettes primitive HamburgLettucePotato Raw LiverChalkBeakersDairy milk sugar TabletWaterSugar Solo Cups Hot PlateKnifeGlovesSkim MilkGlow SticksPeroxide surmise 1. If we smorgasbord the environment via temperature the glitter queer testament Its warmth give change 2. If hydrogen peroxide is played to a trusted food liver then It would blether 3. If a lactaid enzyme to milk the It would divert Procedure (A) 1. Collect three burn sticks. 2.Boil water system in cd mL beaker render 1st glowstick for 30 minutes. 3. run second glowstick in freezer for 30 minutes 4. Leave leash glow stick at live temperature for 30 minutes 5. come upon and magnetic disk findings. Data Collected (A) Place a glow stick in apiece environment below. using a subdue 1-3 1 being normal intensity 3 being brigh sieve intensity BeakerObservation Freezer1 turn Water3 Room Temperature (control)2 Procedure (B) 1. Collect taste of raw liver, potato, raw hamburg, lettuce and starter. 2. Place each(prenominal) sample in individual assay piping in rack. 3. Observe samples former to adding peroxide. 4.Add 1 mL of peroxide to each hear electron furnish 5. Observe and record findings Data Collected (B) answer later on hydrogen peroxide is added. Using a scale of 0-5 0 world no bubbles 5 being the intimately bubbles Test tubeObservation before adding hydrogen peroxideObservation after adding hydrogen peroxide 1 liverDark brownness in tint (expired meat)5 2 potatoInner cutting off with some skin2 3 raw hamburgNormal in color4 4 lettuceFresh green lettuce1 5 chalk Yellow colored chalk0 Procedure (C) zeal 1. Enzyme Solution Add one lactase tablet to 200 ml of water. chide until the tablet is dissolved. . Skim Milk This root contains lactose. 3. Sucrose SolutionAdd 5 grams of sugar to 100 ml of water. erect until the sugar is dissolved. 4. Denatured enzyme Solution 1. Place 20 ml of enzyme answer into a rivulet tube. 2. Add 200 ml of water to a 400 ml beaker. 3. Place the test tube in the beaker. Make sure it does not fail out. 4. Place the beaker and the test tube on a hot plate. 5. Boil the water for 30 minutes. 6. Let the solution coolheaded to room temperature. Procedure (C-1) 1. Collect 6 mL of glidemed milk, do 2mL in each test tube (3 test tubes of skim milk). . Collect 4mL of sucrose solution, place 2mL in each test tube (2 test tubes total). 3. Observe and record sign observations. 4. Add 1mL of enzyme solution to a skim milk test tube add 1mL of water to 2nd skim milk test tube, ad 1mL denatured enzyme solution to 3rd skim milk test tube. 5. Add 1mL of enzyme solution to 1st test tube of sucrose solution add 1mL of water to 2nd test tube of sucrose solution. 6. Insert Glucose test peel in wch test tube (5 total). reside 2 minutes. 7. Ob serve and record whether or not glucose is enter and how lots.Test tubeInitial ObservationGlucose Test strip Is glucose present 1. 2 ml of skim milk and 1ml of the enzyme solutionBubbles3000 Glucose 2. 2 ml of skim milk and 1 ml of waterNo bubbles 2 layers300 Glucose 3. 2 ml of skim milk and 1 ml of denatured solutionBubbles and layers 0 Glucose 4. 2 ml of sucrose solution and 1 ml of enzyme solutionClear liquidYellow in color zero glucose 5. 2 ml of sucrose solution and 1 ml of waterClear liquid Yellow in color no glucose What happens when the enzyme is denatured? The Enzyme does not work. consequent Questions 1. In which beaker did the glow stick glow the most? wherefore do you hazard that is? The beaker that contained hot water. The heat from the water depart speed up the chemical rate of answerion taking place, and therefore will glow brighter the cold one will excite its rate of reaction slowed, and thence be dimmer. 2. What did the glow stick come on about enzymes and different environments? How does changing the temperature impress the rate of an enzyme-controlled experiment? The increasing temperature increases molecular motion and may increase the estimate of times an enzyme contacts and combines with a substrate molecule.Temperature may also influence the shape of the enzyme molecule, qualification it fit better with the substrate. 3. What effect may change in PH take away on an enzyme activity? The three-dimensional social organization of a protein leaves certain side fetter exposed. These side chains may attract ions from the environment. Under the right conditions, a theme of positively charged hydrogen ions may accumulate on certain part of an enzyme. A change in pH disrupts an enzymes shape and structure. When the pH changes an enzymes structure, the enzyme cant do its job. Changes in pH break the delicate bonds that represent an enzymes shape.An enzyme will unravel, or denature, and become vain in a different pH. contrib ute enzymes work in a passing acidic environment of pH 2. A little way down the digestive tract, intestine enzymes need a pH of 8. 4. Why did the hamburger and liver react differently with hydrogen peroxide? They have natural catalase enzymes which were broken down with the heraldic bearing of hydrogen peroxide. 5. What is the job of the lactase enzyme? lactase is an enzyme which breaks down lactose, a sugar tack together in milk and other dairy products. 6. In which test tubes was the glucose present? Why?In test tube 1 the enzyme broke down the lactose . Lactose is a disaccharide of glucose and galactose. The lactose tablet contained lactase which hydrolyzed the lactose into constituent galactose and glucose. Glucose is of course occurring in skim milk thus in test tube 2 found in a much smaller amount than test tube 1. 7. What happened when the enzyme was denatured or altered by heat? Did it work the same? Was glucose present? There was no glucose present. It did not work at all. Summary What the class name today about the jobs of enzymes? What questions does the class appease have?